Characterization of the Vibrio fischeri Fatty Acid Chemoreceptors, VfcB and VfcB2

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Structural and thermodynamic characterization of Vibrio fischeri CcdB.

CcdB(Vfi) from Vibrio fischeri is a member of the CcdB family of toxins that poison covalent gyrase-DNA complexes. In solution CcdB(Vfi) is a dimer that unfolds to the corresponding monomeric components in a two-state fashion. In the unfolded state, the monomer retains a partial secondary structure. This observation correlates well with the crystal and NMR structures of the protein, which show ...

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Characterization of pES213, a small mobilizable plasmid from Vibrio fischeri.

Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjugative system similar to that on plasmid R721. The small plasmids lack extensiv...

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VfcA mediates amino acid chemotaxis in Vibrio fischeri 1 2

23 24 Flagellar motility and chemotaxis by Vibrio fischeri are important behaviors mediating 25 colonization of its mutualistic host, the Hawaiian bobtail squid. However, none of the 43 26 putative methyl-accepting chemotaxis proteins (MCPs) encoded in the V. fischeri genome has 27 been previously characterized. Using both an available transposon mutant collection and 28 directed mutagenesis, w...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 2016

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.02856-15